The TSH-Receptor (TSHR) is a 7-transmembrandomain-receptor, which signals are mainly mediated by a cAMP-signal cascade. The extracellular domain, has epitopes for TSHR-stimulating as well as blocking autoantibodies (autoAb). Die Hyperthyreose in Graves‘ disease caused by TSHR- stimulating autoantibodies (sTRAb). Currently diagnostic assays quantify a pool of TSHR-Ab including blocking Ab indirectly by competition of marked TSH from TSHR by autoantibodiesr (Competition tests). We developed the first “in vitro” test for direct detection of sTRAb suitable for clinical routine diagnostic.


Anchor Proteins:

TSH-Receptor sequence with secretory protein on N-terminus and a specific anchoring sequence for monoclonal antibodies at the C-terminus

Detecting Proteins:

TSH-Receptor sequence with secretory enzyme protein on N-terminus (HRP or SEAP)

Monoclonal Antibodies

Production and purification of monoclonal antibodies from hybridoma cells a an anchor antibody against specific epitope on recombinant proteins


sTRAb is an ELISA based on a bridge technology

  • Detect only TSH-Receptor stimulating Antibodies
  • Direct Epitope Recognition Assay (DERA) – no competition assay
  • High specificity by innovative „Bridge-Assay“ design
  • No falsification by blocking Antibodies
Patient orientated!
  • Basic for correct therapy
  • Early indentification of a remission
  • Minimization of the diagnostic grey area
User friendly!
  • MT-Plate Format
  • High sensitifity by signal increasing chemiluminescence detection
  • Results in less then three hours
Assured of a good future!
  • Basic for the development of automated operating tests on IMMULITE / ADVIA Centaur

„Bridge Technology with TSH Receptor Chimera for Sensitive Direct Detection of TSH Receptor Antibodies Causing Graves’ Disease“