Know How

  • Genetic engineering
  • Establishment of cell line for the production of recombinant proteins by stable gene expression
  • T- and B-cell responses as well as autoantibody profile in autoimmune diseases
  • Development of ELISA to detect autoAb in autoimmune diseases
  • Immunoprecipitation
  • Bridge–Assay for improvement of sensitivity of current ELISA test
  • Immunomagnetic Separation


  • Cloning of customers specific DNA sequences for the expression of recombinant proteins
  • Establishment of cell line for the production of recombinant proteins by stabil gene expression
  • Development of LIA (Luminescence Immuno Assay)
  • Development of Novel Bio-Assays: CRE-Reporter System
    Preparation of reporter gene constructs with customer specific DNA sequences
  • Improvement of existing ELISA-technology by innovative bridge technology.
  • Development of new Assays by bridge technology on paramagnetic beads



Internet Sites
Food and Drug Administration (FDI)
Chemical Abstracts Service
Literature search
Google Scholar
Gene and Protein Data Bases
Sequence Manipulation Suite
Free Software


Orginal Publications

Frank et al., 2015: Bridge Technology with TSH Receptor Chimera for Sensitive Direct Detection of TSH Receptor Antibodies Causing Graves’ Disease: Analytical and Clinical Evaluation. Horm Metab Res 47: 880–888. Download:


Franz et al., 2007: Direct quantification of stimulating TSH receptor autoantibodies in Graves‘ disease by the first in vitro assay suitable for routine clinical diagnosis (Deutsche Gesellschaft für Endokrinologie, Salzburg) Download (2007 DGE_Salzburg.pdf)

Franz et al., 2008: Direct epitope recognition assay for TSH receptor autoantibodies causing Graves’ disease demonstrates higher diagnostic accuracy than indirect assays based on TSH displacement. (American Assocciation of Clinical chemistry, Washington) Download (2008 AACC_Washington.pdf)

Bräth et al., 2015: Assay of TSH receptor stimulating immunoglobulins using paramagnetic microbeads as solid phase (International federation clinical chemistry, Paris) Download (2015 IFCC_Paris.pdf)

Bräth et al., 2015: Novel in vitro chimeric sTRAb assay measures thyroid stimulating autoantibodies (TSI) in serum of Graves’ disease patients (American thyroid assocciation, Orlando) Download (2015 ATA_Orlando.pdf)

Loos et al., 2017: Sera from patients with Hashimoto’s disease impair TSH signaling in a TSH receptor bioassay, and coexisting TRAb are identified as stimulating (Deutsche Gesellschaft für Endokrinologie, Würzburg) Download (2017 DGE_Wuerzburg.pdf)

Secondary Literature

Clinical Evaluation of the IMMULITE 2000 TSI Assay: Kiaei D, Conarpe C, Birmingham N, Lei J, Bitcon V.Siemens Healthcare Diagnostics, Tarrytown, NY, USA Download:
Analytical and Clinical Performance of the IMMULITE 2000 TSI Assay by Kiaei D, Birmingham N, Chapman-Montgomery S, Zhang B, Thompson S, Lei J. Siemens Healthcare, Tarrytown, NY, USA Download: